Recombinant human TNF-alpha protein

TNF, also known as tumor necrosis factor, is cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation. Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/mL.

Molecular Weight

18.1 kDa (163aa)

Additionnal Information

TNF-a, Tumor necrosis factor, DIF, TNF-alpha, TNFA, TNFSF2, ATGP3152-10 µg, ATGP3152-20 µg, ATGP3152-50 µg, ATGP3152-100 µg, ATGP3152-250 µg, ATGP3152-500 µg, ATGP3152-1 mg, ATGP3152-10, ATGP3152-20, ATGP3152-50, ATGP3152-100, ATGP3152-250, ATGP3152-500, ATGP3152-1

References & Citations

Friedmann E. et al., (2006) Nat. Cell Biol. 8:843-848.; ; Zhang XM. et al., (1992) J. Biol. Chem. 267:24069-24075.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_000585.2

Uniprot Accession Number

P01375

Species

Human

AA Sequence

VRSSSRT PSDKPVAHVV ANPQAEGQLQ WLNRRANALL ANGVELRDNQ LVVPSEGLYL IYSQVLFKGQ GCPSTHVLLT HTISRIAVSY QTKVNLLSAI KSPCQRETPE GAEAKPWYEP IYLGGVFQLE KGDRLSAEIN RPDYLDFAES GQVYFGIIAL