Recombinant rat NM23-H2/NME2 protein
Nme2 also known as nucleoside diphosphate kinaseB is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. Nme2 is a ubiquitous enzyme that catalyses phosphorylation of nucleoside 5-diphosphate (NDP) to the corresponding triphosphate (NTP), following a Ping-Pong mechanism which includes the formation of a phosphohistidine intermediate. Recombinant rat Nme2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Nucleoside diphosphate kinase B, NDKB, nm23-2, p18-12d, NME/NM23 nucleoside diphosphate kinase 2, NDP kinase B, Histidine protein kinase NDKB, NM23B
Expression System
E.coli
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
19.7 kDa (175aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ishikawa N., et al. (1992) J. Biol. Chem. 267 (20) :14366-72.; ; Hemmerich S., et al. (1992) Biochemistry. 31 (19) :4574-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 30% glycerol, 0.15M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_114021
Uniprot Accession Number
P19804
Species
Rat
AA Sequence
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