Recombinant human Kallikrein 13/KLK13 protein

KLK13, also known as kallikrein-13, belongs to Kallikrein subfamily. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. KLK13 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Expression of this gene is regulated by steroid hormones and may be useful as a marker for breast cancer. An additional transcript variant has been identified, but its full length sequence has not been determined. Recombinant human KLK13, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 8,000 pmol/min/ug. One unit will hydrolyze 1.0 pmole of BAEE to Na-Benzoyl-L-arginine per minute at pH8.0 at 25C.

Product Name Alternative

Kallikrein related peptidase 13, Kallikrein-13 Kallikrein-like protein 4, KLK-L4

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 8,000pmol/min/ug. One unit will hydrolyze 1.0pmole of BAEE to Na-Benzoyl-L-arginine per minute at pH8.0 at 25C.

Molecular Weight

29.7 kDa (267aa)

Additionnal Information

Kallikrein13, Kallikrein 13, Kallikrein-13, KLK-L4, KLKL4, KLK L4, ATGP3148-10 µg, ATGP3148-20 µg, ATGP3148-50 µg, ATGP3148-100 µg, ATGP3148-250 µg, ATGP3148-500 µg, ATGP3148-1 mg, ATGP3148-010, ATGP3148-020, ATGP3148-050, ATGP3148-100, ATGP3148-250, ATGP3148-500, ATGP3148-01M

References & Citations

Yousef G.M. et al., (2000) J. Biol. Chem. 275:11891-11898.; ; Diamandis EP. et al., (2000) Trends Endocrinol. Metab. 11:54-60.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_056411

Uniprot Accession Number

Q9UKR3

Species

Human

AA Sequence

GGVSQESSKV LNTNGTSGFL PGGYTCFPHS QPWQAALLVQ GRLLCGGVLV HPKWVLTAAH CLKEGLKVYL GKHALGRVEA GEQVREVVHS IPHPEYRRSP THLNHDHDIM LLELQSPVQL TGYIQTLPLS HNNRLTPGTT CRVSGWGTTT SPQVNYPKTL QCANIQLRSD EECRQVYPGK ITDNMLCAGT KEGGKDSCEG DSGGPLVCNR TLYGIVSWGD FPCGQPDRPG VYTRVSRYVL WIRETIRKYE TQQQKWLKGP QHHHHHH