Recombinant mouse Glutathione S-Transferase Pi 1/GSTP1 protein

Gstp1 also known as Glutathione S-transferase P 1. Gstp1 is a superfamily of ubiquitously expressed cytosolic, mitochondrial and membrane-associated enzymes involved in a range of detoxification processes. Also Increased Gstp1 levels were consistently associated with reduced Cdk5 activity. Gstp1 directly inhibits Cdk5 by dislodging p25/p35, and indirectly by eliminating oxidative stress. Recombinant mouse Gstp1, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 40 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S-transferase P 1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 40unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

26 kDa (233aa) confirmed by MALDI-TOF

Additionnal Information

Gstp1, Glutathione S-transferase P 1, ATGP3146-10 µg, ATGP3146-20 µg, ATGP3146-50 µg, ATGP3146-100 µg, ATGP3146-250 µg, ATGP3146-500 µg, ATGP3146-1 mg, ATGP3146-10, ATGP3146-20, ATGP3146-50, ATGP3146-100, ATGP3146-250, ATGP3146-500, ATGP3146-1

References & Citations

Sailer V., et al. (2015) Histopathology. doi: 10.1111/his.12671.; ; S Kai-Hui., et al. (2011) Journal of Neurochemistry vol. 118 (5) : 902-914.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_038569

Uniprot Accession Number

P19157

Species

Mouse

AA Sequence

MPPYTIV YFPVRGRCEA MRMLLADQGQ SWKEEVVTID TWMQGLLKPT CLYGQLPKFE DGDLTLYQSN AILRHLGRSL GLYGKNQREA AQMDMVNDGV EDLRGKYVTL IYTNYENGKN DYVKALPGHL KPFETLLSQN QGGKAFIVGD QISFADYNLL DLLLIHQVLA PGCLDNFPLL SAYVARLSAR PKIKAFLSSP EHVNRPINGN GKQ