Recombinant human Glucosylceramidase/GBA protein
GBA, also known as glucosylceramidase isoform 1, is a lysosomal enzyme that presumably requires a signal peptide for transport across the membrane of the rough endoplasmic reticulum and glycosylation for transport into lysosomes. Gaucher disease is due to a deficiency in the activity of the enzyme glucocerebrosidase. Recombinant human GBA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glucosylceramidase isoform 1, GBA1, GCB, GLUC
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 80% by SDS-PAGE
Molecular Weight
56.4 kDa (503aa)
Additionnal Information
References & Citations
Sorge JA., et al. (1987) Am J Hum Genet. 41:1016-1024.; ; Salvioli R., et al. (2000) FEBS Lett. 472:17-21.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_000148
Uniprot Accession Number
P04062
Species
Human
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