What is the catalog number for Recombinant human GOT2 protein?

The catalog number for Recombinant human GOT2 protein is ATGP3143-004.

What sizes are available for Recombinant human GOT2 protein?

Recombinant human GOT2 protein is available in the following size(s): 4 µg.

Is Recombinant human GOT2 protein in stock?

Recombinant human GOT2 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human GOT2 protein

GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 60 units/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Product Name Alternative

Aspartate aminotransferase 2 mitochondrial, KAT4, KATIV, mitAAT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 60unit/mg, and is defined as the amount of enzyme that convert 1umole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Molecular Weight

47 kDa (424aa) confirmed by MALDI-TOF

Additionnal Information

GOT2, Aspartate aminotransferase 2 mitochondrial, KAT4, KATIV, mitAAT, ATGP3143-10 µg, ATGP3143-20 µg, ATGP3143-50 µg, ATGP3143-100 µg, ATGP3143-250 µg, ATGP3143-500 µg, ATGP3143-1 mg, ATGP3143-10, ATGP3143-20, ATGP3143-50, ATGP3143-100, ATGP3143-250, ATGP3143-500, ATGP3143-1

References & Citations

Guidetti P, Amori L, et al. (2007) . J Neurochem. 102 (1) :103-11.; ; Lain B, Iriarte A, et al. (1995) . J Biol Chem. 270 (42) :24732-9.

Other References

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020) {https://pubmed.ncbi.nlm.nih.gov/31787239/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_002071.2

Uniprot Accession Number

P00505

Species

Human

AA Sequence

SSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK