Recombinant human GOT2 protein
GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. glutamate oxaloacetate transaminas is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Aspartate aminotransferase 2 mitochondrial, KAT4, KATIV, mitAAT
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
47 kDa (424aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Guidetti P, Amori L, et al. (2007) . J Neurochem. 102 (1) :103-11.; ; Lain B, Iriarte A, et al. (1995) . J Biol Chem. 270 (42) :24732-9.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_002071.2
Uniprot Accession Number
P00505
Species
Human
AA Sequence
Frequently Asked Questions
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