Recombinant human Glutathione S-transferase alpha 4/GSTA4 protein

GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S-transferase alpha 4, GSTA4-4, GTA4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

28.3 kDa (246aa) confirmed by MALDI-TOF

Additionnal Information

GSTA4, Glutathione S-transferase alpha 4, GSTA4-4, GTA4, ATGP3141-10 µg, ATGP3141-20 µg, ATGP3141-50 µg, ATGP3141-100 µg, ATGP3141-250 µg, ATGP3141-500 µg, ATGP3141-1 mg, ATGP3141-10, ATGP3141-20, ATGP3141-50, ATGP3141-100, ATGP3141-250, ATGP3141-500, ATGP3141-1

References & Citations

Bruns C.M., et al. (1999) J. Mol. Biol. 288:427-439; ; Balogh L.M., et al. (2010) Biochemistry 49:1541-1548.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 2mM DTT, 20% glycerol,100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_001503

Uniprot Accession Number

O15217

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP