Recombinant human PDIA6 protein

PDIA6 is a member of the protein disulfide isomerase (PDI) . PDI is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. PDIA6 function as a chaperone that inhibits aggregation of misfolded proteins. It plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. Recombinant human PDIA6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Product Name Alternative

Protein disulfide-isomerase A6, ERP5, P5, TXNDC7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Molecular Weight

48.5 kDa (442aa) confirmed by MALDI-TOF

Additionnal Information

PDIA6, Protein disulfide-isomerase A6, ERP5, P5, TXNDC7, ATGP3139-10 µg, ATGP3139-20 µg, ATGP3139-50 µg, ATGP3139-100 µg, ATGP3139-250 µg, ATGP3139-500 µg, ATGP3139-1 mg, ATGP3139-10, ATGP3139-20, ATGP3139-50, ATGP3139-100, ATGP3139-250, ATGP3139-500, ATGP3139-1

References & Citations

Jordan P.A., et al. (2005) Blood. 105:1500-1507; ; Kikuchi M., et al. (2002) J. Biochem. 132:451-455

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 50 mM NaCl, 2mM DTT and 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_005733

Uniprot Accession Number

Q15084

Species

Human

AA Sequence

LYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL