Recombinant human PDIA4 protein
PDIA4, also known as ERP72, is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. It is involved in the catalysis of protein-S-S-bond rearrangement. PDIA3 and PDIA4 act as proteases, protein disulfide isomerases, phospholipases or a combination of these. Recombinant human PDIA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
Product Name Alternative
Protein disulfide-isomerase A4, Endoplasmic reticulum resident protein 72, ERP70, ERP72
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
Molecular Weight
72.9 kDa (646aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Thomas M., et al. (2010) Synapse. 64 (8) :579-93.; ; Joo JH., et al. (2007) Cancer Res. 67 (16) :7929-36.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl,1mM DTT and 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_004902.1
Uniprot Accession Number
P13667
Species
Human
AA Sequence
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