Recombinant human GRP58/PDIA3 protein

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Product Name Alternative

Protein disulfide isomerase family A member 3, Glucose regulated protein 58kDa, GPR58, Protein disulfide isomerase-associated 3, 58 kDa microsomal protein, P58, ERp61, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, GRP57, PI-PLC, HsT17083

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Molecular Weight

58.5 kDa (518aa) confirmed by MALDI-TOF

Additionnal Information

ERp57, PDIA3, Protein disulfide-isomerase A3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, ATGP3137-10 µg, ATGP3137-20 µg, ATGP3137-50 µg, ATGP3137-100 µg, ATGP3137-250 µg, ATGP3137-500 µg, ATGP3137-1 mg, ATGP3137-10, ATGP3137-20, ATGP3137-50, ATGP3137-100, ATGP3137-250, ATGP3137-500, ATGP3137-1

References & Citations

Vigneron N., et al. (2009) Eur J Immunol. 39 (9) :2371-6. ; ; Forster ML., et al. (2009) J Biol Chem. 284 (19) :13045-56.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,0.1 M NaCl, and 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_005304

Uniprot Accession Number

P30101

Species

Human

AA Sequence

MSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL