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Recombinant mouse Acy1 protein

Acy1 also known as Aminoacylase1. The protein is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 has been assigned to chromosome 3p21. 1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. Recombinant mouse Acy1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Aminoacylase 1, ACY-1, Acy1, ACY1_HUMAN, ACY1D, ACYLASE, Acylase I, Aminoacylase-1, EC 3.5.1.14, epididymis secretory protein Li 5, HEL-S-5, N acyl L amino acid amidohydrolase, N-acyl-L-amino-acid amidohydrolase, OTTHUMP00000212459, OTTHUMP00000212462, OTTHUMP00000212463, OTTHUMP00000212464, OTTHUMP00000212465

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.4 kDa (433aa) confirmed by MALDI-TOF

Additionnal Information

Acy1, Aminoacylase 1, ACY-1, Acy1 , ACY1_HUMAN , ACY1D , ACYLASE , Acylase I , Aminoacylase-1 , EC 3.5.1.14 , epididymis secretory protein Li 5 , HEL-S-5 , N acyl L amino acid amidohydrolase , N-acyl-L-amino-acid amidohydrolase , OTTHUMP00000212459 , OTTHUMP00000212462 , OTTHUMP00000212463 , OTTHUMP00000212464 , OTTHUMP00000212465, ATGP3131-10 µg, ATGP3131-20 µg, ATGP3131-50 µg, ATGP3131-100 µg, ATGP3131-250 µg, ATGP3131-500 µg, ATGP3131-1 mg, ATGP3131-10, ATGP3131-20, ATGP3131-50, ATGP3131-100, ATGP3131-250, ATGP3131-500, ATGP3131-1

References & Citations

Miller YE, Drabkin H, et al. (1991) Genomics 8 (1) : 149-154. ; ; Voss R, et al. (1982) Ann Hum Genet 44 (Pt 1) : 1-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

AAH05631

Uniprot Accession Number

Q99JW2

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMTTKD PESEHPSVTL FRQYLRICTV QPNPDYGGAI TFLEERARQL GLSCQKIEVV PGFVITVLTW PGTNPSLPSI LLNSHTDVVP VFKEHWHHDP FEAFKDSEGY IYARGSQDMK SVSIQYLEAV RRLKSEGHRF PRTIHMTFVP DEEVGGHKGM ELFVKRPEFQ ALRAGFALDE GLANPTDAFT VFYSERSPWW VRVTSTGKPG HASRFIEDTA AEKLHKVISS ILAFREKERQ RLQANPHLKE GAVTSVNLTK LEGGVAYNVV PATMSASFDF RVAPDVDMKA FEKQLQRWCQ EAGEGVTFEF AQKFTEPRMT PTDDSDPWWA AFSGACKAMN LTLEPEIFPA ATDSRYIRAV GIPALGFSPM NRTPVLLHDH NERLHEDIFL RGVDIYTGLL SALASVPTLP GES

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