Recombinant human Carbonic Anhydrase 12/CA12 protein

CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Product Name Alternative

Carbonic anhydrase 12 isoform 1, CAXII, HsT18816, Carbonate dehydratase XII, CA-XII, tumor antigen HOM-RCC-3.1.3, Carbonic Anhydrase XII

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Molecular Weight

31.94 kDa (283aa)

Additionnal Information

Carbonic Anhydrase XII, Carbonic anhydrase 12 isoform 1, CAXII, HsT18816, ATGP3124-10 µg, ATGP3124-20 µg, ATGP3124-50 µg, ATGP3124-100 µg, ATGP3124-250 µg, ATGP3124-500 µg, ATGP3124-1 mg, ATGP3124-10, ATGP3124-20, ATGP3124-50, ATGP3124-100, ATGP3124-250, ATGP3124-500, ATGP3124-1