Recombinant human JNK1 protein

MAPK8 also known as mitogen-activated protein kinase 8 JNK beta 2. MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. Recombinant human MAPK8, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase 8 JNK beta 2, JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 85% by SDS-PAGE

Molecular Weight

50.5 kDa (450aa)

Additionnal Information

JNK1, Mitogen-activated protein kinase 8 JNK beta 2, JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c, ATGP3120-10 µg, ATGP3120-20 µg, ATGP3120-50 µg, ATGP3120-100 µg, ATGP3120-250 µg, ATGP3120-500 µg, ATGP3120-1 mg, ATGP3120-10, ATGP3120-20, ATGP3120-50, ATGP3120-100, ATGP3120-250, ATGP3120-500, ATGP3120-1

References & Citations

Jha A., et al. (2014) EMBO J. 33 (5), 501-511; ; Misheva M., et al. (2014) Biochim. Biophys. Acta 1843 (2), 253-264;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001265476

Uniprot Accession Number

P45983

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSRSKRD NNFYSVEIGD STFTVLKRYQ NLKPIGSGAQ GIVCAAYDAI LERNVAIKKL SRPFQNQTHA KRAYRELVLM KCVNHKNIIG LLNVFTPQKS LEEFQDVYIV MELMDANLCQ VIQMELDHER MSYLLYQMLC GIKHLHSAGI IHRDLKPSNI VVKSDCTLKI LDFGLARTAG TSFMMTPYVV TRYYRAPEVI LGMGYKENVD IWSVGCIMGE MIKGGVLFPG TDHIDQWNKV IEQLGTPCPE FMKKLQPTVR TYVENRPKYA GYSFEKLFPD VLFPADSEHN KLKASQARDL LSKMLVIDAS KRISVDEALQ HPYINVWYDP SEAEAPPPKI PDKQLDEREH TIEEWKELIY KEVMDLEERT KNGVIRGQPS PLGAAVINGS QHPSSSSSVN DVSSMSTDPT LASDTDSSLE AAAGPLGCCR