Recombinant human Trappin-2/Elafin protein
PI3, also known as elafin or peptidase inhibitor 3, is neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Recombinant human PI3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Elafin, ESI, SKALP, WAP3, WFDC14
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.2 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
10.7 kDa (101aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_002629
Uniprot Accession Number
P19957
Species
Human
AA Sequence
AVTGVPVKGQ DTVKGRVPFN GQDPVKGQVS VKGQDKVKAQ EPVKGPVSTK PGSCPIILIR CAMLNPPNRC LKDTDCPGIK KCCEGSCGMA CFVPQHHHHH H
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