Recombinant human Cysteine Conjugate beta -Lyase/CCBL1 protein

CCBL1 also known as Kynurenine--oxoglutrate transaminase1 isoform a. CCBL1 catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA) and it metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. CCBL1 catalyzes the beta-elimination of S-conjugates and Se-conjugates of L- (seleno) cysteine, resulting in the cleavage of the C-S or C-Se bond. Recombinant human CCBL1 was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Kynurenine-oxoglutrate transaminase1 isoform a, GTK, KAT1, KATI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

50.3 kDa (445aa)

Additionnal Information

Cysteine Conjugate b-Lyase, CCBL1, Kynurenine-oxoglutrate transaminase1 isoform a, GTK, KAT1, KATI, ATGP3102-10 µg, ATGP3102-20 µg, ATGP3102-50 µg, ATGP3102-100 µg, ATGP3102-250 µg, ATGP3102-500 µg, ATGP3102-1 mg, ATGP3102-10, ATGP3102-20, ATGP3102-50, ATGP3102-100, ATGP3102-250, ATGP3102-500, ATGP3102-1

References & Citations

Han Q., et al. (2009) J. Med. Chem. 52:2786-2793.; ; Rossi F., et al. (2004) J. Biol. Chem. 279:50214-50220.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_004050

Uniprot Accession Number

Q16773

Species

Human

AA Sequence

MAKQLQA RRLDGIDYNP WVEFVKLASE HDVVNLGQGF PDFPPPDFAV EAFQHAVSGD FMLNQYTKTF GYPPLTKILA SFFGELLGQE IDPLRNVLVT VGGYGALFTA FQALVDEGDE VIIIEPFFDC YEPMTMMAGG RPVFVSLKPG PIQNGELGSS SNWQLDPMEL AGKFTSRTKA LVLNTPNNPL GKVFSREELE LVASLCQQHD VVCITDEVYQ WMVYDGHQHI SIASLPGMWE RTLTIGSAGK TFSATGWKVG WVLGPDHIMK HLRTVHQNSV FHCPTQSQAA VAESFEREQL LFRQPSSYFV QFPQAMQRCR DHMIRSLQSV GLKPIIPQGS YFLITDISDF KRKMPDLPGA VDEPYDRRFV KWMIKNKGLV AIPVSIFYSV PHQKHFDHYI RFCFVKDEAT LQAMDEKLRK WKVEL