Recombinant human MIF protein

MIF also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Macrophage migration inhibitory factor, GIF, GLIF, MMIF

Expression System

E.coli

Antigen Species

Human

Tag

GST-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

39.2 kDa (345aa) confirmed by MALDI-TOF

Additionnal Information

MIF, ATGP3101-10 µg, ATGP3101-20 µg, ATGP3101-50 µg, ATGP3101-100 µg, ATGP3101-250 µg, ATGP3101-500 µg, ATGP3101-1 mg, ATGP3101-10, ATGP3101-20, ATGP3101-50, ATGP3101-100, ATGP3101-250, ATGP3101-500, ATGP3101-1

References & Citations

Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.; ; Bucala R., et al. (1996) FASEB J 10: 1607-13.

Other References

Garai J, et al. Tetralone derivatives are MIF tautomerase inhibitors and attenuate macrophage activation and amplify the hypothermic response in endotoxemic mice. (J Enzyme Inhib Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/34225560/}