Recombinant human QTRT1 protein

QTRT1 also known as queuine tRNA-ribosyltransferase. QTRT1 interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7- (((4, 5-cis-dihydroxy-2-cyclopenten-1-yl) amino) methyl) -7-deazaguanosine) . Recombinant QTRT1 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Queuine tRNA-ribosyltransferase, FP3235, TGT, TGUT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

46.7 kDa (428aa) confirmed by MALDI-TOF

Additionnal Information

QTRT1, Queuine tRNA-ribosyltransferase, FP3235, TGT, TGUT, ATGP3099-10 µg, ATGP3099-20 µg, ATGP3099-50 µg, ATGP3099-100 µg, ATGP3099-250 µg, ATGP3099-500 µg, ATGP3099-1 mg, ATGP3099-10, ATGP3099-20, ATGP3099-50, ATGP3099-100, ATGP3099-250, ATGP3099-500, ATGP3099-1

References & Citations

Deshpande K.L., et al. (2001) Gene 265:205-212

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_112486

Uniprot Accession Number

Q9BXR0

Species

Human

AA Sequence

MAGAA TQASLESAPR IMRLVAECSR SRARAGELWL PHGTVATPVF MPVGTQATMK GITTEQLDAL GCRICLGNTY HLGLRPGPEL IQKANGLHGF MNWPHNLLTD SGGFQMVSLV SLSEVTEEGV RFRSPYDGNE TLLSPEKSVQ IQNALGSDII MQLDDVVSST VTGPRVEEAM YRSIRWLDRC IAAHQRPDKQ NLFAIIQGGL DADLRATCLE EMTKRDVPGF AIGGLSGGES KSQFWRMVAL STSRLPKDKP RYLMGVGYAT DLVVCVALGC DMFDCVFPTR TARFGSALVP TGNLQLRKKV FEKDFGPIDP ECTCPTCQKH SRAFLHALLH SDNTAALHHL TVHNIAYQLQ LMSAVRTSIV EKRFPDFVRD FMGAMYGDPT LCPTWATDAL ASVGITLG