Recombinant mouse MIF protein
Mif also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant mouse Mif, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
Macrophage migration inhibitory factor, GIF, Glif
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
14.9 kDa (138aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Miyatake S., et al. (2014) Biochem. Biophys. Res. Commun. 444 (4), 496-501; ; Muller I., et al. (2013) J. Biol. Chem. 288 (44), 31635-31645
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_034928
Uniprot Accession Number
P34884
Species
Mouse
AA Sequence
Frequently Asked Questions
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