Recombinant mouse MIF protein

Mif also known as Macrophage migration inhibitory factor. MIF has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant mouse Mif, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Macrophage migration inhibitory factor, GIF, Glif

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

14.9 kDa (138aa) confirmed by MALDI-TOF

Additionnal Information

MIF, Macrophage migration inhibitory factor, GIF, Glif, ATGP3091-10 µg, ATGP3091-20 µg, ATGP3091-50 µg, ATGP3091-100 µg, ATGP3091-250 µg, ATGP3091-500 µg, ATGP3091-1 mg, ATGP3091-10, ATGP3091-20, ATGP3091-50, ATGP3091-100, ATGP3091-250, ATGP3091-500, ATGP3091-1

References & Citations

Miyatake S., et al. (2014) Biochem. Biophys. Res. Commun. 444 (4), 496-501; ; Muller I., et al. (2013) J. Biol. Chem. 288 (44), 31635-31645

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_034928

Uniprot Accession Number

P34884

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPMFIVN TNVPRASVPE GFLSELTQQL AQATGKPAQY IAVHVVPDQL MTFSGTNDPC ALCSLHSIGK IGGAQNRNYS KLLCGLLSDR LHISPDRVYI NYYDMNAANV GWNGSTFA