Recombinant mouse PGP9.5/UCHL1 protein

Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Recombinant mouse Uchl1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase isozyme L1, AW822034, C88048, gad, PGP 9.5, PGP9.5, R75593, UCH-L1, UCHL-1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.2 kDa (246aa) confirmed by MALDI-TOF

Additionnal Information

UCH-L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, AW822034, C88048, gad, PGP 9.5, PGP9.5, R75593, UCH-L1, UCHL-1, ATGP3089-10 µg, ATGP3089-20 µg, ATGP3089-50 µg, ATGP3089-100 µg, ATGP3089-250 µg, ATGP3089-500 µg, ATGP3089-1 mg, ATGP3089-10, ATGP3089-20, ATGP3089-50, ATGP3089-100, ATGP3089-250, ATGP3089-500, ATGP3089-1

References & Citations

Wiese CB., et al. (2013) Genesis 51 (12), 852-861

Other References

Almer G, et al. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. Biologics. 2011;5:95-105.{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195669}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_035800

Uniprot Accession Number

Q9R0P9

Species

Mouse

AA Sequence

MQLKPME INPEMLNKVL AKLGVAGQWR FADVLGLEEE TLGSVPSPAC ALLLLFPLTA QHENFRKKQI EELKGQEVSP KVYFMKQTIG NSCGTIGLIH AVANNQDKLE FEDGSVLKQF LSETEKLSPE DRAKCFEKNE AIQAAHDSVA QEGQCRVDDK VNFHFILFNN VDGHLYELDG RMPFPVNHGA SSEDSLLQDA AKVCREFTER EQGEVRFSAV ALCKAA