Recombinant mouse Glutathione S-transferase A1/GSTA1 protein

Gsta1 also known as Glutathione S-transferase, A1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant mouse Gsta1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S-transferase, A1, Gst2-1, OTTMUSG00000031890

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 4,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

28 kDa (246aa) confirmed by MALDI-TOF

Additionnal Information

Gsta1, Glutathione S-transferase A1, Glutathione S-transferase, A1, Gst2-1, OTTMUSG00000031890, ATGP3086-10 µg, ATGP3086-20 µg, ATGP3086-50 µg, ATGP3086-100 µg, ATGP3086-250 µg, ATGP3086-500 µg, ATGP3086-1 mg, ATGP3086-10, ATGP3086-20, ATGP3086-50, ATGP3086-100, ATGP3086-250, ATGP3086-500, ATGP3086-1

References & Citations

Grahn, E., et al. (2006) Acta Cryst. 62 (2) : 197-207.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_032207

Uniprot Accession Number

P13745

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKD GNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQR EAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSR ISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ