Recombinant mouse AST1/GOT1 protein

Got1 also known as Glutamate oxaloacetate transaminase 1 is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant mouse Got1 fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 80 units/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Product Name Alternative

Transaminase A, Glutamate oxaloacetate transaminase 1 soluble, Glutamate oxaloacetate transaminase 1, Cytoplasmic, Cysteine transaminase, Cysteine aminotransferase, cCAT, cAspAT

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 80unit/mg, and is defined as the amount of enzyme that converts 1.0 micromole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

Molecular Weight

48.6 kDa (436aa) confirmed by MALDI-TOF

Additionnal Information

Got1, Glutamate oxaloacetate transaminase 1 soluble, cAspAT, ATGP3085-10 µg, ATGP3085-20 µg, ATGP3085-50 µg, ATGP3085-100 µg, ATGP3085-250 µg, ATGP3085-500 µg, ATGP3085-1 mg, ATGP3085-10, ATGP3085-20, ATGP3085-50, ATGP3085-100, ATGP3085-250, ATGP3085-500, ATGP3085-1

References & Citations

Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

AAH02057

Uniprot Accession Number

P05201

Species

Mouse

AA Sequence

MAPPSVF AQVPQAPPVL VFKLTADFRD DPDPRKVNLG VGAYRTDESQ PWVLPVVRKV EQKIANDNSL NHEYLPILGL AEFRSCASRL VLGDNSPAIR ENRVGGVQSL GGTGALRIGA DFLGRWYNGT DNKNTPIYVS SPTWENHNAV FSAAGFKDIR PYCYWDAEKR GLDLQGFLND LENAPEFSIF VLHACAHNPT GTDPTPEQWK QIAAVMQRRF LFPFFDSAYQ GFASGDLEKD AWAIRYFVSE GFELFCAQSF SKNFGLYNER VGNLTVVGKE SDSVLRVLSQ MEKIVRITWS NPPAQGARIV AATLSDPELF KEWKGNVKTM ADRILTMRSE LRARLEALKT PGTWSHITEQ IGMFSFTGLN PKQVEYLVNE KHIYLLPSGR INMCGLTTKN LDYVATSIHE AVTKIQ