Recombinant mouse FKBP12/FKBP1A protein
Fkbp1a also known as peptidyl-prolyl cis-trans isomerase FKBP1A belongs to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Fkbp1a also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1, 4, 5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant mouse Fkbp1a, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.4 kDa (132aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chen H., et al. (2013) Development 140 (9), 1946-1957
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_032045
Uniprot Accession Number
P26883
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMGVQVE TISPGDGRTF PKRGQTCVVH YTGMLEDGKK FDSSRDRNKP FKFTLGKQEV IRGWEEGVAQ MSVGQRAKLI ISSDYAYGAT GHPGIIPPHA TLVFDVELLK LE
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