Recombinant human IL-17A protein

IL17A, also known as Interleukin 17, is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation. It is produced by T-helper cells and is induced by IL23 which results in destructive tissue damage in delayed-type reactions. This protein is a 155 amino acid protein that is a disulfide-linked, homodimeric, secreted glycoprotein with a molecular mass of 35kDa. The structure of IL17A consists of a signal peptide of 23 amino acids followed by a 123 aa chain region characteristic of the IL17 family. An N-linked glycosylation site on the protein was first identified after purification of the protein revealed two bands, one at 15kDa and another at 20kDa. Recombinant human IL17A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IL-17A stimulates IL-6 secretion of the NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 7 ng/mL.

Product Name Alternative

Interleukin 17A, IL-17, IL-17A, IL17, CTLA8, Cytotoxic T-lymphocyte-associated protein 8, Cytotoxic T-lymphocyte-associated serine esterase 8

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 7 ng/mL.

Molecular Weight

15.9 kDa (138aa)

Additionnal Information

IL17A, Interleukin 17A, CTLA8, IL-17, IL-17A, IL17, ATGP3083-10 µg, ATGP3083-20 µg, ATGP3083-50 µg, ATGP3083-100 µg, ATGP3083-250 µg, ATGP3083-500 µg, ATGP3083-1 mg, ATGP3083-10, ATGP3083-20, ATGP3083-50, ATGP3083-100, ATGP3083-250, ATGP3083-500, ATGP3083-1