Recombinant human HMGCS1 protein

HMGCS1 also known as hydroxymethylglutaryl-CoA synthase, cytoplasmic. This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. Recombinant human HMGCS1 was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Hydroxymethylglutaryl-CoA synthase cytoplasmic, Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMGCS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

59.7 kDa (543aa)

Additionnal Information

HMGCS1, Hydroxymethylglutaryl-CoA synthase cytoplasmic, Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMGCS, ATGP3079-10 µg, ATGP3079-20 µg, ATGP3079-50 µg, ATGP3079-100 µg, ATGP3079-250 µg, ATGP3079-500 µg, ATGP3079-1 mg, ATGP3079-10, ATGP3079-20, ATGP3079-50, ATGP3079-100, ATGP3079-250, ATGP3079-500, ATGP3079-1

References & Citations

Rigbolt K.T., et al. (2011) Sci. Signal. 4:RS3-RS3.; ; Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001091742

Uniprot Accession Number

Q01581

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMPGSLPL NAEACWPKDV GIVALEIYFP SQYVDQAELE KYDGVDAGKY TIGLGQAKMG FCTDREDINS LCMTVVQNLM ERNNLSYDCI GRLEVGTETI IDKSKSVKTN LMQLFEESGN TDIEGIDTTN ACYGGTAAVF NAVNWIESSS WDGRYALVVA GDIAVYATGN ARPTGGVGAV ALLIGPNAPL IFERGLRGTH MQHAYDFYKP DMLSEYPIVD GKLSIQCYLS ALDRCYSVYC KKIHAQWQKE GNDKDFTLND FGFMIFHSPY CKLVQKSLAR MLLNDFLNDQ NRDKNSIYSG LEAFGDVKLE DTYFDRDVEK AFMKASSELF SQKTKASLLV SNQNGNMYTS SVYGSLASVL AQYSPQQLAG KRIGVFSYGS GLAATLYSLK VTQDATPGSA LDKITASLCD LKSRLDSRTG VAPDVFAENM KLREDTHHLV NYIPQGSIDS LFEGTWYLVR VDEKHRRTYA RRPTPNDDTL DEGVGLVHSN IATEHIPSPA KKVPRLPATA AEPEAAVISN GEH