Recombinant mouse PGK1 protein

Pgk1 also known as Phosphoglycerate kinase 1 is an X-linked enzyme that plays a key role in the glycolytic pathway. Pgk1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. This protein catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant mouse Pgk1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Product Name Alternative

Phosphoglycerate kinase 1, Pgk-1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity: > 500unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Molecular Weight

47.1 kDa (441aa) confirmed by MALDI-TOF

Additionnal Information

Pgk1, Phosphoglycerate kinase 1, Pgk-1, ATGP3072-10 µg, ATGP3072-20 µg, ATGP3072-50 µg, ATGP3072-100 µg, ATGP3072-250 µg, ATGP3072-500 µg, ATGP3072-1 mg, ATGP3072-10, ATGP3072-20, ATGP3072-50, ATGP3072-100, ATGP3072-250, ATGP3072-500, ATGP3072-1

References & Citations

Chauvin T., et al. (2012) Biol. Reprod. 87 (6), 141

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_032854

Uniprot Accession Number

P09411

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V