Recombinant human PYCR2 protein

PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

36 kDa (343aa) confirmed by MALDI-TOF

Additionnal Information

PYCR2, Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2, ATGP3066-10 µg, ATGP3066-20 µg, ATGP3066-50 µg, ATGP3066-100 µg, ATGP3066-250 µg, ATGP3066-500 µg, ATGP3066-1 mg, ATGP3066-10, ATGP3066-20, ATGP3066-50, ATGP3066-100, ATGP3066-250, ATGP3066-500, ATGP3066-1

References & Citations

De Ingeniis J, et al. (2012) . PLoS One. 7 (9) :e45190.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 5mM DTT, 1mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_037460

Uniprot Accession Number

Q96C36

Species

Human

AA Sequence

MSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRK MGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKV IRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDA LADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINA VEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD