Recombinant human SNX5 protein

SNX5 also known as sorting nexin-5, is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein is a component of the mammalian retromer complex, which facilitates cargo retrieval from endosomes to the trans-Golgi network. It has also been shown to bind to the Fanconi anemia, complementation group A protein. Recombinant human SNX5, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sorting nexin-5 isoform a, Sorting nexin 5, snx 5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

49.2 kDa (427aa) confirmed by MALDI-TOF

Additionnal Information

SNX5, Sorting nexin-5 isoform a, Sorting nexin 5, snx 5, ATGP3065-10 µg, ATGP3065-20 µg, ATGP3065-50 µg, ATGP3065-100 µg, ATGP3065-250 µg, ATGP3065-500 µg, ATGP3065-1 mg, ATGP3065-10, ATGP3065-20, ATGP3065-50, ATGP3065-100, ATGP3065-250, ATGP3065-500, ATGP3065-1

References & Citations

Wassmer T., et al. (2007) J Cell Sci. 120:45-54; ; Otsuki T., et al. (1999) Biochem Biophys Res Commun. 265:630-635.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_689413

Uniprot Accession Number

Q9Y5X3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAVPEL LQQQEEDRSK LRSVSVDLNV DPSLQIDIPD ALSERDKVKF TVHTKTTLPT FQSPEFSVTR QHEDFVWLHD TLIETTDYAG LIIPPAPTKP DFDGPREKMQ KLGEGEGSMT KEEFAKMKQE LEAEYLAVFK KTVSSHEVFL QRLSSHPVLS KDRNFHVFLE YDQDLSVRRK NTKEMFGGFF KSVVKSADEV LFTGVKEVDD FFEQEKNFLI NYYNRIKDSC VKADKMTRSH KNVADDYIHT AACLHSLALE EPTVIKKYLL KVAELFEKLR KVEGRVSSDE DLKLTELLRY YMLNIEAAKD LLYRRTKALI DYENSNKALD KARLKSKDVK LAEAHQQECC QKFEQLSESA KEELINFKRK RVAAFRKNLI EMSELEIKHA RNNVSLLQSC IDLFKNN