Recombinant human PYGL protein
PYGL also known as Glycogen phosphorylase, switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. Recombinant human PYGL, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glycogen phosphorylase liver form isoform 1, Glycogen phosphorylase, liver form isoform 1, GSD6
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
100.7 kDa (879aa)
Additionnal Information
References & Citations
Tomihira M. et al. (2004) . Diabetes Res Clin Pract. 65:175-182
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_002854
Uniprot Accession Number
P06737
Species
Human
AA Sequence
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