Recombinant mouse Annexin A5/ANXA5 protein

Anxa5 also known as Annexin A5. Annexin A5, belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Anxa5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Recombinant mouse Anxa5, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Vascular anticoagulant-alpha, VAC-alpha, Thromboplastin inhibitor, PP4, Placental anticoagulant protein I, PAP I, Lipocortin V, ENX2, Endonexin II, CPB-I, Calphobindin I, ANX5, ANX A5, Annexin V, Annexin A5, Annexin 5, Anchorin CII

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

38.1 kDa (342aa) confirmed by MALDI-TOF

Additionnal Information

Annexin V, Annexin A5, Anx5, R74653, ATGP3060-10 µg, ATGP3060-20 µg, ATGP3060-50 µg, ATGP3060-100 µg, ATGP3060-250 µg, ATGP3060-500 µg, ATGP3060-1 mg, ATGP3060-10, ATGP3060-20, ATGP3060-50, ATGP3060-100, ATGP3060-250, ATGP3060-500, ATGP3060-1

References & Citations

Jeong JJ., et al. (2014) J. Biol. Chem. 289 (4), 2469-2481; ; Liu BC., et al. (2013) Am. J. Physiol. Renal Physiol. 305 (3), F304-F313;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_033803.1

Uniprot Accession Number

P48036

Species

Mouse

AA Sequence

MATRGTV TDFPGFDGRA DAEVLRKAMK GLGTDEDSIL NLLTSRSNAQ RQEIAQEFKT LFGRDLVDDL KSELTGKFEK LIVAMMKPSR LYDAYELKHA LKGAGTDEKV LTEIIASRTP EELSAIKQVY EEEYGSNLED DVVGDTSGYY QRMLVVLLQA NRDPDTAIDD AQVELDAQAL FQAGELKWGT DEEKFITIFG TRSVSHLRRV FDKYMTISGF QIEETIDRET SGNLEQLLLA VVKSIRSIPA YLAETLYYAM KGAGTDDHTL IRVVVSRSEI DLFNIRKEFR KNFATSLYSM IKGDTSGDYK KALLLLCGGE DD