Recombinant humanCarbonic Anhydrase 13/CA13 protein

CA13 also known as carbonic anhydrase 13 belongs to the alpha-carbonic anhydrase family. The carbonic anhydrase from a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that occurs relatively slowly in the absence of catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are claasified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta, and epsilon) . These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Recombinant human CA13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Product Name Alternative

Carbonic anhydrase 13, CAXIII, Carbonic anhydrase XIII, Carbonate dehydratase XIII

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Molecular Weight

31.8 kDa (285aa) confirmed by MALDI-TOF

Additionnal Information

CAXIII, CA 13, CA XIII, CA13, Carbonate dehydratase 13, Carbonate dehydratase XIII, Carbonate dehydratase13, Carbonate dehydrataseXIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, Carbonic anhydrase13, Carbonic anhydraseXIII, ATGP3059-10 µg, ATGP3059-20 µg, ATGP3059-50 µg, ATGP3059-100 µg, ATGP3059-250 µg, ATGP3059-500 µg, ATGP3059-1 mg, ATGP3059-010, ATGP3059-020, ATGP3059-050, ATGP3059-100, ATGP3059-250, ATGP3059-500, ATGP3059-01M

References & Citations

Lamesch P., et al. (2007) Genomics 89 (3), 307-315

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_940986.1

Uniprot Accession Number

Q8N1Q1

Species

Human

AA Sequence

MSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH