Recombinant mouse Apolipoprotein A1/APOA1 protein

Apoa1 also known as Apolipoprotein A-I, is the major protein component of high density lipoprotein (HDL) in plasma. This protein promotes cholesterol efflux from tissues to the liver for excretion. And it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apoa1 are associated with HDL deficiency including Tangier disease. Recombinant mouse Apoa1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Apolipoprotein A-I, Alp-1, apo-AI, Apoa-1, apoA-I, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, Apolipoprotein AI

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.3 kDa (263aa) confirmed by MALDI-TOF

Additionnal Information

Apolipoprotein A-I, Apolipoprotein A-I, Alp-1, apo-AI, Apoa-1, apoA-I, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, Apolipoprotein AI, ATGP3058-10 µg, ATGP3058-20 µg, ATGP3058-50 µg, ATGP3058-100 µg, ATGP3058-250 µg, ATGP3058-500 µg, ATGP3058-1 mg, ATGP3058-10, ATGP3058-20, ATGP3058-50, ATGP3058-100, ATGP3058-250, ATGP3058-500, ATGP3058-1

References & Citations

Sontag TJ., et al. (2014) Arterioscler Thromb Vasc Biol, 34 (2), 262-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_033822.2

Uniprot Accession Number

Q00623

Species

Mouse

AA Sequence

DEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ