Recombinant human MICA protein
MICA also known as MHC class I polypeptide-related sequence A. This Protein's Function seems to have no role in antigen presentation. It acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. It binding to KLRK1 leads to cell lysis. Recombinant human MICA, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human MICA is coated at 10 µg/mL (100 ul/well) can bind Human NKG2D (CAT# ATGP3662) in a Functional ELISA assay.
Product Name Alternative
MHC class I polypeptide-related sequence A, MHC class I polypeptide-related sequence A, PERB11.1, truncated
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human NKG2D (CAT# ATGP3662)
Molecular Weight
32.7 kDa (283aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Strausberg, R.L, et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99 (26), 16899-16903
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol
Scientific Category
Immunology
NCBI Accession Number
AAH16929.1
Uniprot Accession Number
Q29983
Species
Human
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