Recombinant human MAGEA8 protein

MAGEA8 also known as Melanoma-associated antigen 8 is a member of the MAGE gene family, which comprises 12 known genes, of which 6 are expressed in tumors. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. Recombinant human MAGEA8, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Melanoma-associated antigen 8, CT1.8, MAGE8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37.6 kDa (341aa) confirmed by MALDI-TOF

Additionnal Information

MAGEA8, Melanoma-associated antigen 8, CT1.8, MAGE8, ATGP3052-10 µg, ATGP3052-20 µg, ATGP3052-50 µg, ATGP3052-100 µg, ATGP3052-250 µg, ATGP3052-500 µg, ATGP3052-1 mg, ATGP3052-10, ATGP3052-20, ATGP3052-50, ATGP3052-100, ATGP3052-250, ATGP3052-500, ATGP3052-1

References & Citations

Serrano A., et al. (1999) Int. J. Cancer 83 (5), 664-669; ; Rogner uC., et al. (1995) Genomics 29 (3), 725-731;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_001159873

Uniprot Accession Number

P43361

Species

Human

AA Sequence

MLLGQKS QRYKAEEGLQ AQGEAPGLMD VQIPTAEEQK AASSSSTLIM GTLEEVTDSG SPSPPQSPEG ASSSLTVTDS TLWSQSDEGS SSNEEEGPST SPDPAHLESL FREALDEKVA ELVRFLLRKY QIKEPVTKAE MLESVIKNYK NHFPDIFSKA SECMQVIFGI DVKEVDPAGH SYILVTCLGL SYDGLLGDDQ STPKTGLLII VLGMILMEGS RAPEEAIWEA LSVMGLYDGR EHSVYWKLRK LLTQEWVQEN YLEYRQAPGS DPVRYEFLWG PRALAETSYV KVLEHVVRVN ARVRISYPSL HEEALGEEKG V