Recombinant human AMT protein

AMT also known as Aminomethyltransferase, mitochondrial isoform 1. AMT is a component of the glycine cleavage system termed T-protein, reversibly catalyzes the degradation of the aminomethyl moiety of glycine attached to the lipoate cofactor of H-protein, resulting in the production of ammonia, 5, 10-methylenetetrahydrofolate, and dihydrolipoate-bearing H-protein in the presence of tetrahydrofolate. Recombinant human AMT was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Aminomethyltransferase mitochondrial isoform 1, Aminomethyltransferase, mitochondrial isoform 1, GCE, GCST, GCVT, NKH

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

43.3 kDa (398aa) confirmed by MALDI-TOF

Additionnal Information

AMT, Aminomethyltransferase mitochondrial isoform 1, Aminomethyltransferase, mitochondrial isoform 1, GCE, GCST, GCVT, NKH, ATGP3051-10 µg, ATGP3051-20 µg, ATGP3051-50 µg, ATGP3051-100 µg, ATGP3051-250 µg, ATGP3051-500 µg, ATGP3051-1 mg, ATGP3051-10, ATGP3051-20, ATGP3051-50, ATGP3051-100, ATGP3051-250, ATGP3051-500, ATGP3051-1

References & Citations

Narisawa A., et al. (2012) Hum. Mol. Genet. 21 (7), 1496-1503; ; Kure S., et al. (2006) Hum. Mutat. 27 (4), 343-352;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Neuroscience

NCBI Accession Number

NP_000472

Uniprot Accession Number

P48728

Species

Human

AA Sequence

AQEVLRR TPLYDFHLAH GGKMVAFAGW SLPVQYRDSH TDSHLHTRQH CSLFDVSHML QTKILGSDRV KLMESLVVGD IAELRPNQGT LSLFTNEAGG ILDDLIVTNT SEGHLYVVSN AGCWEKDLAL MQDKVRELQN QGRDVGLEVL DNALLALQGP TAAQVLQAGV ADDLRKLPFM TSAVMEVFGV SGCRVTRCGY TGEDGVEISV PVAGAVHLAT AILKNPEVKL AGLAARDSLR LEAGLCLYGN DIDEHTTPVE GSLSWTLGKR RRAAMDFPGA KVIVPQLKGR VQRRRVGLMC EGAPMRAHSP ILNMEGTKIG TVTSGCPSPS LKKNVAMGYV PCEYSRPGTM LLVEVRRKQQ MAVVSKMPFV PTNYYTLK