Recombinant human PRKAR2A protein

PRKAR2A also known as cAMP-dependent protein kinase type II-alpha regulatory subunit. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. Recombinant human PRKAR2A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

48.6 kDa (434aa) confirmed by MALDI-TOF

Additionnal Information

PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2, ATGP3050-10 µg, ATGP3050-20 µg, ATGP3050-50 µg, ATGP3050-100 µg, ATGP3050-250 µg, ATGP3050-500 µg, ATGP3050-1 mg, ATGP3050-10, ATGP3050-20, ATGP3050-50, ATGP3050-100, ATGP3050-250, ATGP3050-500, ATGP3050-1

References & Citations

Yang H., et al. (2013) J. Biol. Chem. 288 (12), 8737-8749

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004148

Uniprot Accession Number

P13861

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSHIQIP PGLTELLQGY TVEVLRQQPP DLVEFAVEYF TRLREARAPA SVLPAATPRQ SLGHPPPEPG PDRVADAKGD SESEEDEDLE VPVPSRFNRR VSVCAETYNP DEEEEDTDPR VIHPKTDEQR CRLQEACKDI LLFKNLDQEQ LSQVLDAMFE RIVKADEHVI DQGDDGDNFY VIERGTYDIL VTKDNQTRSV GQYDNRGSFG ELALMYNTPR AATIVATSEG SLWGLDRVTF RRIIVKNNAK KRKMFESFIE SVPLLKSLEV SERMKIVDVI GEKIYKDGER IITQGEKADS FYIIESGEVS ILIRSRTKSN KDGGNQEVEI ARCHKGQYFG ELALVTNKPR AASAYAVGDV KCLVMDVQAF ERLLGPCMDI MKRNISHYEE QLVKMFGSSV DLGNLGQ