Recombinant human NUBP1 protein
NUBP1 also known as cytosolic Fe-S cluster assembly factor NUBP1 isoform 1. NUBP1 is implicated in the regulation of centrosome duplication by similarity. This protein is component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. It required for maturation of extra mitochondrial Fe-S proteins The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of a Fe-S cluster and its transfer to target apoproteins. Recombinant human NUBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytosolic NUBP iron-sulfur cluster assembly factor 1, Cytosolic Fe-S cluster assembly factor NUBP1, Nucleotide-binding protein 1, NBP1, NBP35, CIAO5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.9 kDa (343aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Stehling O., et al. (2008) Mol. Cell. Biol. 28:5517-5528.; ; Bienvenut W.V., et al. (2012) Mol. Cell. Proteomics 11:M111.015131-M111.015131;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002475.2
Uniprot Accession Number
P53384
Species
Human
AA Sequence
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