Recombinant human NUBP1 protein

NUBP1 also known as cytosolic Fe-S cluster assembly factor NUBP1 isoform 1. NUBP1 is implicated in the regulation of centrosome duplication by similarity. This protein is component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. It required for maturation of extra mitochondrial Fe-S proteins The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of a Fe-S cluster and its transfer to target apoproteins. Recombinant human NUBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytosolic NUBP iron-sulfur cluster assembly factor 1, Cytosolic Fe-S cluster assembly factor NUBP1, Nucleotide-binding protein 1, NBP1, NBP35, CIAO5

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.9 kDa (343aa) confirmed by MALDI-TOF

Additionnal Information

NuBP1, Cytosolic Fe-S cluster assembly factor NuBP1 isoform 1, Cytosolic Fe-S cluster assembly factor NuBP1 isoform 1, NBP, NBP1, NBP35, ATGP3046-10 µg, ATGP3046-20 µg, ATGP3046-50 µg, ATGP3046-100 µg, ATGP3046-250 µg, ATGP3046-500 µg, ATGP3046-1 mg, ATGP3046-10, ATGP3046-20, ATGP3046-50, ATGP3046-100, ATGP3046-250, ATGP3046-500, ATGP3046-1

References & Citations

Stehling O., et al. (2008) Mol. Cell. Biol. 28:5517-5528.; ; Bienvenut W.V., et al. (2012) Mol. Cell. Proteomics 11:M111.015131-M111.015131;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002475.2

Uniprot Accession Number

P53384

Species

Human

AA Sequence

MEEVPHD CPGADSAQAG RGASCQGCPN QRLCASGAGA TPDTAIEEIK EKMKTVKHKI LVLSGKGGVG KSTFSAHLAH GLAEDENTQI ALLDIDICGP SIPKIMGLEG EQVHQSGSGW SPVYVEDNLG VMSVGFLLSS PDDAVIWRGP KKNGMIKQFL RDVDWGEVDY LIVDTPPGTS DEHLSVVRYL ATAHIDGAVI ITTPQEVSLQ DVRKEINFCR KVKLPIIGVV ENMSGFICPK CKKESQIFPP TTGGAELMCQ DLEVPLLGRV PLDPLIGKNC DKGQSFFIDA PDSPATLAYR SIIQRIQEFC NLHQSKEENL ISS