Recombinant human Sonic Hedgehog/Shh (C24II) protein
SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers. Recombinant human SHH was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Sonic hedgehog protein, Sonic hedgehog protein, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
19.8 kDa (176aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Richieri-Costa A., et al. (2006) Am. J. Med. Genet. A 140:2587-2593.; ; El-Jaick K.B., et al (2005) Am. J. Med. Genet. A 136:345-345.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_000184
Uniprot Accession Number
Q15465
Species
Human
AA Sequence
Frequently Asked Questions
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