Recombinant human Sonic Hedgehog/Shh (C24II) protein

SHH, also known as Sonic hedgehog protein, is one of three proteins in the mammalian signaling pathway family called hedgehog. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. It controls cell division of adult stem cells and has been implicated in development of some cancers. Recombinant human SHH was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sonic hedgehog protein, Sonic hedgehog protein, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.8 kDa (176aa) confirmed by MALDI-TOF

Additionnal Information

Sonic Hedgehog, Shh (C24II), Sonic hedgehog protein, Sonic hedgehog protein, HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS, ATGP3037-10 µg, ATGP3037-20 µg, ATGP3037-50 µg, ATGP3037-100 µg, ATGP3037-250 µg, ATGP3037-500 µg, ATGP3037-1 mg, ATGP3037-10, ATGP3037-20, ATGP3037-50, ATGP3037-100, ATGP3037-250, ATGP3037-500, ATGP3037-1

References & Citations

Richieri-Costa A., et al. (2006) Am. J. Med. Genet. A 140:2587-2593.; ; El-Jaick K.B., et al (2005) Am. J. Med. Genet. A 136:345-345.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000184

Uniprot Accession Number

Q15465

Species

Human

AA Sequence

MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG