Recombinant human LAP3 protein
LAP3 also known as cytosol aminopeptidase. LAP3 presumably involved in the processing and regular turnover of intracellular proteins. This protein catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. It release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Recombinant human LAP3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytosol aminopeptidase, HEL-S-106, LAP, LAPEP, PEPS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
58.3 kDa (539aa)
Additionnal Information
References & Citations
Hendrickson SL., et al. (2010) PLoS ONE 5 (9), E12862
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 50% glycerol, 5mM DTT, 1mM EDTA
Scientific Category
Metabolism
NCBI Accession Number
NP_056991.2
Uniprot Accession Number
P28838
Species
Human
AA Sequence
Frequently Asked Questions
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