Recombinant human PNPT1 protein
PNPT1 also known as polyribonucleotide nucleotidyltransferase 1. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. Recombinant human PNPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Polyribonucleotide nucleotidyltransferase 1, Polyribonucleotide nucleotidyltransferase 1, COXPD13, DFNB70, old-35, OLD35, PNPASE
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
83.3 kDa (761aa)
Additionnal Information
References & Citations
Leszczyniecka M., et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99:16636-16641; ; Sarkar D., et al. (2003) J. Biol. Chem. 278:24542-24551;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_149100.2
Uniprot Accession Number
Q8TCS8
Species
Human
AA Sequence
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