Recombinant human PNPT1 protein

PNPT1 also known as polyribonucleotide nucleotidyltransferase 1. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. Recombinant human PNPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Polyribonucleotide nucleotidyltransferase 1, Polyribonucleotide nucleotidyltransferase 1, COXPD13, DFNB70, old-35, OLD35, PNPASE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

83.3 kDa (761aa)

Additionnal Information

PNPT1, Polyribonucleotide nucleotidyltransferase 1, Polyribonucleotide nucleotidyltransferase 1, COXPD13, DFNB70, old-35, OLD35, PNPASE, ATGP3024-10 µg, ATGP3024-20 µg, ATGP3024-50 µg, ATGP3024-100 µg, ATGP3024-250 µg, ATGP3024-500 µg, ATGP3024-1 mg, ATGP3024-10, ATGP3024-20, ATGP3024-50, ATGP3024-100, ATGP3024-250, ATGP3024-500, ATGP3024-1

References & Citations

Leszczyniecka M., et al. (2002) Proc. Natl. Acad. Sci. u.S.A. 99:16636-16641; ; Sarkar D., et al. (2003) J. Biol. Chem. 278:24542-24551;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_149100.2

Uniprot Accession Number

Q8TCS8

Species

Human

AA Sequence

AVAVDLG NRKLEISSGK LARFADGSAV VQSGDTAVMV TAVSKTKPSP SQFMPLVVDY RQKAAAAGRI PTNYLRREIG TSDKEILTSR IIDRSIRPLF PAGYFYDTQV LCNLLAVDGV NEPDVLAING ASVALSLSDI PWNGPVGAVR IGIIDGEYVV NPTRKEMSSS TLNLVVAGAP KSQIVMLEAS AENILQQDFC HAIKVGVKYT QQIIQGIQQL VKETGVTKRT PQKLFTPSPE IVKYTHKLAM ERLYAVFTDY EHDKVSRDEA VNKIRLDTEE QLKEKFPEAD PYEIIESFNV VAKEVFRSIV LNEYKRCDGR DLTSLRNVSC EVDMFKTLHG SALFQRGQTQ VLCTVTFDSL ESGIKSDQVI TAINGIKDKN FMLHYEFPPY ATNEIGKVTG LNRRELGHGA LAEKALYPVI PRDFPFTIRV TSEVLESNGS SSMASACGGS LALMDSGVPI SSAVAGVAIG LVTKTDPEKG EIEDYRLLTD ILGIEDYNGD MDFKIAGTNK GITALQADIK LPGIPIKIVM EAIQQASVAK KEILQIMNKT ISKPRASRKE NGPVVETVQV PLSKRAKFVG PGGYNLKKLQ AETGVTISQV DEETFSVFAP TPSAMHEARD FITEICKDDQ EQQLEFGAVY TATITEIRDT GVMVKLYPNM TAVLLHNTQL DQRKIKHPTA LGLEVGQEIQ VKYFGRDPAD GRMRLSRKVL QSPATTVVRT LNDRSSIVMG EPISQSSSNS Q