Recombinant human CRMP1 protein

CRMP1 also known as Dihydropyrimidinase-related protein 1 isoform2. It is necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. It plays a role in axon guidance, invasive growth and cell migration and may participate in cytokinesis. Recombinant human CRMP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, CRMP-1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3, ULIP-3, DPYSL1, ULIP3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

64.8 kDa (597aa) Multiple bands

Additionnal Information

Unc-33-like phosphoprotein 3, Unc33 like phosphoprotein 3, Unc 33 like phosphoprotein 3, ULIP-3, ULIP3, ULIP 3, Inactive dihydropyrimidinase, DRP-1, DRP1, DRP 1, DPYSL-1, DPYSL1, DPYSL 1, Dihydropyrimidinase-related protein 1, Dihydropyrimidinase related protein 1, CRMP-1, CRMP1, CRMP 1, Collapsin response mediator protein 1, ATGP3019-10 µg, ATGP3019-20 µg, ATGP3019-50 µg, ATGP3019-100 µg, ATGP3019-250 µg, ATGP3019-500 µg, ATGP3019-1 mg, ATGP3019-010, ATGP3019-020, ATGP3019-050, ATGP3019-100, ATGP3019-250, ATGP3019-500, ATGP3019-01M

References & Citations

Shih J.-Y. et al. (2001) J. Natl. Cancer Inst. 93:1392-1400.; ; Chen T.C. et al. (2009) J. Proteome Res. 8:4943-4953.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT, 0.2mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_001304

Uniprot Accession Number

Q14194

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMSYQG KKSIPHITSD RLLIKGGRII NDDQSLYADV YLEDGLIKQI GENLIVPGGV KTIEANGRMV IPGGIDVNTY LQKPSQGMTA ADDFFQGTRA ALVGGTTMII DHVVPEPGSS LLTSFEKWHE AADTKSCCDY SLHVDITSWY DGVREELEVL VQDKGVNSFQ VYMAYKDVYQ MSDSQLYEAF TFLKGLGAVI LVHAENGDLI AQEQKRILEM GITGPEGHAL SRPEELEAEA VFRAITIAGR INCPVYITKV MSKSAADIIA LARKKGPLVF GEPIAASLGT DGTHYWSKNW AKAAAFVTSP PLSPDPTTPD YLTSLLACGD LQVTGSGHCP YSTAQKAVGK DNFTLIPEGV NGIEERMTVV WDKAVATGKM DENQFVAVTS TNAAKIFNLY PRKGRIAVGS DADVVIWDPD KLKTITAKSH KSAVEYNIFE GMECHGSPLV VISQGKIVFE DGNINVNKGM GRFIPRKAFP EHLYQRVKIR NKVFGLQGVS RGMYDGPVYE VPATPKYATP APSAKSSPSK HQPPPIRNLH QSNFSLSGAQ IDDNNPRRTG HRIVAPPGGR SNITSLG