Recombinant human AgRP protein
AGRP, also known as agouti-related protein also called agouti-related peptide, is a neuropeptide produced in the brain by the AGRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. AGRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators. Recombinant human AGRP, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Agouti-related protein, Agouti-related protein, AGRT, ART, ASIP2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.1 kDa (137aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Xiao E, Xia-Zhang L, et al. (2003) . Endocrinology. 144 (5) :1736-41.; ; Creemers JW, Pritchard LE, et al. (2006) . Endocrinology. 147 (4) :1621-31.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 20% glycerol, 1mM DTT
Scientific Category
Cardiovascular
NCBI Accession Number
NP_001129
Uniprot Accession Number
O00253
Species
Human
AA Sequence
AQMGL APMEGIRRPD QALLPELPGL GLRAPLKKTT AEQAEEDLLQ EAQALAEVLD LQDREPRSSR RCVRLHESCL GQQVPCCDPC ATCYCRFFNA FCYCRKLGTA MNPCSRT
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