Recombinant human ACP6 protein

ACP6 also known as Iysophosphatidic acid phosphatase type 6, is Hydrolyzes lysophosphatidic acid (LPA) containing a medium length fatty acid chain to the corresponding monoacylglycerol. Has highest activity with lysophosphatidic acid containing myristate (C14:0), monounsaturated oleate (C18:1) or palmitate (C16:0), and lower activity with C18:0 and C6:0 lysophosphatidic acid. Recombinant human ACP6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 70 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Product Name Alternative

Lysophosphatidic acid phosphatase type 6, lysophosphatidic acid phosphatase type 6, ACPL1, LPAP, PACPL1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Molecular Weight

47.7 kDa (419aa)

Additionnal Information

ACP6, lysophosphatidic acid phosphatase type 6, lysophosphatidic acid phosphatase type 6, ACPL1, LPAP, PACPL1, ATGP3015-10 µg, ATGP3015-20 µg, ATGP3015-50 µg, ATGP3015-100 µg, ATGP3015-250 µg, ATGP3015-500 µg, ATGP3015-1 mg, ATGP3015-10, ATGP3015-20, ATGP3015-50, ATGP3015-100, ATGP3015-250, ATGP3015-500, ATGP3015-1

References & Citations

Hiroyama M., et al. (1999) J. Biol. Chem. 274:29172-29180.; ; Li J., et al. (2013) Protein Cell 4:548-561.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057445.4

Uniprot Accession Number

Q9NPH0

Species

Human

AA Sequence

ELQEADG QCPVDRSLLK LKMVQVVFRH GARSPLKPLP LEEQVEWNPQ LLEVPPQTQF DYTVTNLAGG PKPYSPYDSQ YHETTLKGGM FAGQLTKVGM QQMFALGERL RKNYVEDIPF LSPTFNPQEV FIRSTNIFRN LESTRCLLAG LFQCQKEGPI IIHTDEADSE VLYPNYQSCW SLRQRTRGRR QTASLQPGIS EDLKKVKDRM GIDSSDKVDF FILLDNVAAE QAHNLPSCPM LKRFARMIEQ RAVDTSLYIL PKEDRESLQM AVGPFLHILE SNLLKAMDSA TAPDKIRKLY LYAAHDVTFI PLLMTLGIFD HKWPPFAVDL TMELYQHLES KEWFVQLYYH GKEQVPRGCP DGLCPLDMFL NAMSVYTLSP EKYHALCSQT QVMEVGNEE