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Recombinant human PDGF-B protein

PDGFB, also known as, Platelet-derived growth factor subunit B isoform 2, is a member of Platelet derived growth factor (PDGF) family. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. Recombinant human PDGFB, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Platelet-derived growth factor subunit B isoform 2, Platelet-derived growth factor subunit B isoform 2, c-sis, IBGC5, PDGF-2, PDGF2, SIS, SSV

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

14.7 kDa (130aa) confirmed by MALDI-TOF

Additionnal Information

PDGF-B, Platelet-derived growth factor subunit B isoform 2, Platelet-derived growth factor subunit B isoform 2, c-sis, IBGC5, PDGF-2, PDGF2, SIS, SSV, ATGP3007-10 µg, ATGP3007-20 µg, ATGP3007-50 µg, ATGP3007-100 µg, ATGP3007-250 µg, ATGP3007-500 µg, ATGP3007-1 mg, ATGP3007-10, ATGP3007-20, ATGP3007-50, ATGP3007-100, ATGP3007-250, ATGP3007-500, ATGP3007-1

References & Citations

Van Steensel L., et al. (2012) J Clin Endocrinol Metab. 97 (6) : 37062-37071.; ; Malabanan KP, et al. (2012) Am J Pathol. 180 (6) : 2590-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_148937.1

Uniprot Accession Number

P01127

Species

Human

AA Sequence

CKTRTEVFE ISRRLIDRTN ANFLVWPPCV EVQRCSGCCN NRNVQCRPTQ VQLRPVQVRK IEIVRKKPIF KKATVTLEDH LACKCETVAA ARPVTRSPGG SQEQRAKTPQ

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