Recombinant mouse IL-18 protein

IL18 (Interleukin18), previously known as interferon-gamma (IFN-gamma) -inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. Recombinant mouse IL18 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Interleukin 18, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Igif

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.2 kDa (158aa) confirmed by MALDI-TOF

Additionnal Information

Interleukin-18, Igif, Il-18, IL18, IL1F4, Interleukin-1 gamma, IL-1 gamma, ATGP3006-10 µg, ATGP3006-20 µg, ATGP3006-50 µg, ATGP3006-100 µg, ATGP3006-250 µg, ATGP3006-500 µg, ATGP3006-1 mg, ATGP3006-010, ATGP3006-020, ATGP3006-050, ATGP3006-100, ATGP3006-250, ATGP3006-500, ATGP3006-01M

References & Citations

Yoshimoto T., et al. (1998) J. Immunol, 161, 3400. ; ; Gracie JA ., et al. (2003) J Leukoc Biol. 73,213.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_032386

Uniprot Accession Number

P70380

Species

Mouse

AA Sequence

MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS