Recombinant human Transglutaminase 2/TGM2 protein

TGM2 also known as Protein-glutamine gamma-glutamyltransferase 2. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Recombinant human TGM2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein-glutamine gamma-glutamyltransferase 2 isoform a, Transglutaminase 2, G-ALPHA-h, GNAH, HEL-S-45, TG2; TGC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

79.7 kDa (710aa)

Additionnal Information

Transglutaminase2, TGM2, Protein-glutamine gamma-glutamyltransferase 2 isoform a, Transglutaminase 2, G-ALPHA-h, GNAH, HEL-S-45, TG2; TGC, ATGP3003-10 µg, ATGP3003-20 µg, ATGP3003-50 µg, ATGP3003-100 µg, ATGP3003-250 µg, ATGP3003-500 µg, ATGP3003-1 mg, ATGP3003-10, ATGP3003-20, ATGP3003-50, ATGP3003-100, ATGP3003-250, ATGP3003-500, ATGP3003-1

References & Citations

Jeong JH., et al. (2013) J. Korean Med. Sci. 28 (7), 1005-1014

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_004604

Uniprot Accession Number

P21980

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEELVL ERCDLELETN GRDHHTADLC REKLVVRRGQ PFWLTLHFEG RNYEASVDSL TFSVVTGPAP SQEAGTKARF PLRDAVEEGD WTATVVDQQD CTLSLQLTTP ANAPIGLYRL SLEASTGYQG SSFVLGHFIL LFNAWCPADA VYLDSEEERQ EYVLTQQGFI YQGSAKFIKN IPWNFGQFED GILDICLILL DVNPKFLKNA GRDCSRRSSP VYVGRVVSGM VNCNDDQGVL LGRWDNNYGD GVSPMSWIGS VDILRRWKNH GCQRVKYGQC WVFAAVACTV LRCLGIPTRV VTNYNSAHDQ NSNLLIEYFR NEFGEIQGDK SEMIWNFHCW VESWMTRPDL QPGYEGWQAL DPTPQEKSEG TYCCGPVPVR AIKEGDLSTK YDAPFVFAEV NADVVDWIQQ DDGSVHKSIN RSLIVGLKIS TKSVGRDERE DITHTYKYPE GSSEEREAFT RANHLNKLAE KEETGMAMRI RVGQSMNMGS DFDVFAHITN NTAEEYVCRL LLCARTVSYN GILGPECGTK YLLNLNLEPF SEKSVPLCIL YEKYRDCLTE SNLIKVRALL VEPVINSYLL AERDLYLENP EIKIRILGEP KQKRKLVAEV SLQNPLPVAL EGCTFTVEGA GLTEEQKTVE IPDPVEAGEE VKVRMDLLPL HMGLHKLVVN FESDKLKAVK GFRNVIIGPA